Standard transmitted light objective lenses are designed for a 0.17mm cover slip between the sample and the lens. Standard cover slips have a thickness of 0.13-0.16mm, taking into consideration the added layer of an embedding medium or water into account in the sample results in meeting the rule of 0.17mm.
The main function of the cover slip is to keep solid specimens pressed flat and liquid samples shaped into a flat layer of even thickness. This is necessary because high-resolution microscopes have a very narrow region within their focus.
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