Oxoid Chromogenic Coliform Agar (CCA) is a selective, differential medium designed for the detection and enumeration of E. coli and coliform bacteria in water and food samples. It provides clear results in just 24 hours without the need for subculturing. Purchase or review the dehydrated powder via Thermo Fisher Scientific.
Key Features and Principle
The medium relies on two specific chromogenic substrates to differentiate target organisms, typically Salmon GAL and X-glucuronide.
Total Coliforms: Cleave the Salmon GAL substrate, resulting in distinct pink to red colonies.
E. coli: Produces both β-D-galactosidase and β-D-glucuronidase enzymes. This combination cleaves both chromogens, creating dark blue to violet colonies.
Selectivity: It contains Tergitol-7 to inhibit Gram-positive bacteria and non-coliform Gram-negative bacteria